Characterization and Anti-proliferative Activity of Ethanol Extract of Tartary Buckwheat

Full Text (PDF, 275KB), PP.7-12

Views: 0 Downloads: 0


Yuxiang Ma 1,* Benguo Liu 2 Huirong Zhang 2 Li Yang 2

1. Henan University of Technology, Zhengzhou 510640, China

2. Henan Institute of Science and Technology, Xinxiang 453003, China

* Corresponding author.


Received: 12 Oct. 2010 / Revised: 23 Nov. 2010 / Accepted: 30 Dec. 2010 / Published: 8 Feb. 2011

Index Terms

Tartary buckwheat, extraction, antioxidant, apoptosis


Tartary buckwheat (Fagopyrum tataricum) has been consumed in China as health food and medicine. In this study, the ethanol extract (EE) of tartary buckwheat was prepared. The major flavonoid in EE was identified as rutin by HPLC-PDA-ESI/MS and the content of rutin in EE was determined as 27.4 % by HPLC. In the anticancer assay, it was found EE could inhibit the proliferation of the human hepatocellular liver carcinoma Hep G2 cell line in a dose-dependent manner. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining, which suggested that EE could induce Hep G2 cells to enter into apoptosis.

Cite This Paper

Yuxiang Ma, Benguo Liu, Li Yang, Huirong Zhang,"Characterization and Anti-proliferative Activity of Ethanol Extract of Tartary Buckwheat", IJEM, vol.1, no.1, pp.7-12, 2011. DOI: 10.5815/ijem.2011.01.02


[1] N. Fabjan, J. Rode, I.J. Košir, Z. Wang, Z. Zhang, I. Kreft, “Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitrin,” J. Agric. Food Chem, 2003, vol. 51, pp. 6452-6455.

[2] G. Bonafaccia, M. Marocchini, I. Kreft, “Composition and technological properties of the flour and bran from common and tartary buckwheat,” Food Chem, 2003, vol. 80, pp. 9-15.

[3] H. Kitabayashi, A. Ujihara, T. Hirose, M. Minami, “On the genotypic differences for rutin content in tatary buckwheat, Fagopyrum tataricum Gaertn,” Breeding Sci, 1995, vol. 45, pp. 189-194.

[4] T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity assays,” J. Immunol Methods, 1983, vol. 65, pp.55-63.

[5] B. Liu, Y. Zhu, “Extraction of flavonoids from flavonoid-rich parts in tartary buckwheat and identification of the main flavonoids,” J. Food Eng, 2007, vol. 78, pp. 584-587.

[6] M.D. Jacobson, M. Weil, M.C. Raff, “Programmed cell death in animal development,” Cell, 1997, vol. 88, pp. 347-354.

[7] S. Nagata, “Apoptosis by death factor,” Cell, 1997, vol. 88, pp. 355-365.

[8] V.J. Kidd, “Proteolytic activities that mediate apoptosis,” Annu. Rev. Physiol, 1998, vol. 60, pp. 533-573.

[9] C.B. Thompson, “Apoptosis in the pathogenesis and treatment of disease,” Science, 1995, vol. 267, pp. 1456-1462.

[10] M. Van Engeland, L.J.W. Nieland, F.C.S. Ramaekers, B. Schutte, C.P.M. Reutelingsperger, “Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure,” Cytometry, 1998, vol. 31, pp. 1-9.